The intended purpose herein was to obtain RA dissolved in primary crystallization mother liquor. During this salting out process, RA could be precipitated from MLS. This was a mass transfer process that should be affected by temperature. Temperature has two effects on the mass transfer process. Increasing temperature can prompt the molecular movement and thus increase the mass transfer rate. At the same time, it can increase the solubility of the substance but reduce solid yield Rebaudioside M, (also known as Rebaudioside X; CAS No: 1220616-44-3) is one of minor steviol glycosides found in Stevia rebaudiana plant. It was found to have superior taste properties and is a highly desirable natural high intensity sweetener (WO2013/096420 007748 incorporated herein as a reference, in its entirety). The present invention is a method for purifying Rebaudioside C by subjecting "waste material," generated during the Rebaudioside A manufacturing process, to liquid-liquid extraction and recrystallizing the Rebaudioside C. Herein, we propose an integrated membrane system, implementing two different ultrafiltration membranes (100 and 1 kDa), to separate rebaudioside A (reb A) from aqueous extracts of Stevia rebaudiana. By using this approach, we found out that the UF100 membrane served mostly to remove total solids (∼42%) and carbohydrates (∼41%) from the crude extract, while tight UF1 membrane was able to retain the phenolic compounds (∼98%). Moreover, the integrated membrane system allowed In this work, an integrated membrane system consisting in two ultrafiltration processes (100 and 1 kDa) was proposed as an alternative for the simultaneous extraction and recovery of rebaudioside A, carbohydrates and polyphenols from Stevia rebaudiana. The final color of the resulting extract (1-Permeate) was reduced by ∼300%. While the 100 kDa membrane allowed to remove high content of solids and carbohydrates, which promote the reduction of fouling in the subsequent process The preparative separation process of rebaudioside A with macroporous resins was developed in this work. The present investigation revealed that the application of DOE using Taguchi approach facilitated the process optimization by understanding the role of factors involved in the rebaudioside A yield. Furthermore, I also demonstrated that HPD-400 as resin was sufficient to increase of rebaudioside A yield under the conditions tested. The starting material is purified stevia leaf extract, produced from Stevia rebaudiana Bertoni (stevia) leaves using the traditional hot water extraction process. Blue California's product contains no less than 85% rebaudioside E and no less than 95% total steviol glycosides. This preparation is designated as high purity rebaudioside E (≥85% The yield in rebaudioside A having a more value as comparing with ethanol 30% which confirmed that the combination of aqueous two-phase separation with ultrasound-assisted extraction improved the extraction of rebaudioside A. Compared with classical methods like maceration and heat extraction, the utilization of ultrasound-assisted extraction proved to be a much simpler and more effective mean to obtain efficiently extractive species from plants. Also, the ultrasound-assisted A new improvised process of extraction of steviosides from the stevia leaves is established in which the dry treated leaves were grounded, defatted, and extracted through pressurized hot wate