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33342) was used to counterstain the cell nuclei (blue fluorescence). Learn more. IHC Troubleshooting Guide · Immunochemistry (IHC) vs Immunocytochemistry (ICC) This guide illustrates the basic steps used to create an IHC stain. Get Knowledge Pathway updates delivered directly to your inbox. Subscribe today! Learn everything from the basics through to advanced techniques and troubleshooting for immunohistochemistry (IHC) with our complete expert guide. A beginners guide to Immunohistochemistry · 1. Tissue preparation · 2. Tissue Preparation: Other things to try · 3. Antigen retrieval · 4. Sample handling · 5. Easily plan and optimize your IHC experiments with our comprehensive guide. Download your free guide now ​. The booklet includes tips and guidelines on: Sample This brief guide is intended to serve as a reference for researchers to understand, perform, and troubleshoot various IHC protocols during the development and Immunohistochemistry (IHC) combines histological, immunological and biochemical techniques for the identification of specific tissue components by means of4 Immunohistochemistry Selection Guide. 6 Pioneering in IHC Technology. 8 Choosing a Detection System. 10 Avidin-Biotin (ABC) Based Detection.