qiaquick gel extraction kit protocol pdf
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gel extraction protocol pdf

This protocol is designed to extract and purify DNA of 70 bp to 10 kb from standard or low-melt agarose gels in TAE or TBE buffer. Up to 400 mg agarose can The GeneJET™ Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either This handbook describes the use of gel filtration for the purification and is the elution volume of molecules that are excluded from the gel filtration. Protocol for Gel Purification with OMEGA Gel Extraction Kit. 1. Perform agarose gel/ethidium bromide electrophoresis to fractionate DNA fragments. The GenElute Gel Extraction Kit is designed for the rapid purification of 50 bp to 10 Kb linear DNA fragments and plasmids from standard or low-melting agarose QIAquick® PCR & Gel Cleanup Kit. The QIAquick Gel Extraction Kit and the QIAquick PCR & Gel Cleanup Kit (cat. nos. QIAGEN kit handbook or user manual. Washing eliminates salts and residual agarose gel. High-purity DNA fragments are eluted with provided EL Buffer (10. mM Tris-Cl, pH 8.5) or distilled water.Quick (15 minute) high-yield recovery of ultra-pure DNA from agarose gels. • Column design permits DNA elution at high concentrations into minimal volumes Operation Time: 20 minutes for gel extraction, 10 minutes for PCR cleanup. Elution Volume: 20-50 l. Kit Storage: dry at room temperature (15-25ºC).