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This manual provides an overview of the Gateway® Technology and provides instructions and guidelines to: 1. Design attB PCR primers and amplify your sequence of Gateway® LR Clonase® II enzyme mix catalyzes the in vitro recombination between an entry clone All usage must comply with product instructions.A new enzyme (LR Clonase® II Plus) is supplied in this kit, and the MultiSite. Gateway® LR recombination reaction protocol has been changed. Follow the protocol 3. Verify PCR insert by restriction digest, PCR, and/or DNA sequencing. 4. Clone insert into destination vector(s) using LR Clonase Gateway® LR Clonase™ II enzyme mix is a proprietary enzyme and buffer formulation containing the bacteriophage lambda recombination proteins. B. Transfer gene from Entry Clone into Destination Vector with LR CLONASE Enzyme GATEWAY Cloning Technology Instruction Manual, but does not provide